Molecular Analysis of Cancer

Author : Jacqueline Boultwood & Carrie Fidler

Published in : Humana press

ISBN : 0-89603-622-7

File type : pdf

File Size : 2 mb

Language : English

Description

Over the past 20 years, technological advances in molecular biology have proven invaluable to the understanding of the pathogenesis of human cancer. The application of molecular technology to the study of cancer has not only led to advances in tumor diagnosis, but has also provided markers for the assessment of prognosis and disease progression. The aim of Molecular Analysis of Cancer is to provide a comprehensive collection of the most up-to-date techniques for the detection of molecular changes in human cancer. Leading researchers in the field have contributed chapters detailing practical procedures for a wide range of state-of-the-art techniques. Molecular Analysis of Cancer includes chapters describing techniques for the identification of chromosomal abnormalities and comprising: fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), and microsatellite analysis. FISH has a prominent role in the molecular analysis of cancer and can be used for the detection of numerical and structural chromosomal abnormalities. The recently described SKY, in which all human metaphase chromosomes are visualized in specific colors, allows for the definition of all chromosomal rearrangements and marker chromosomes in a tumor cell. Protocols for the detection of chromosomal rearrangements by PCR and RT-PCR are described, as well as the technique of DNA fingerprinting, a powerful tool for studying somatic genetic alterations in tumorigenesis. A number of approaches to identify mutations are detailed, and include SSCP, DGGE, the nonisotopic RNase cleavage assay, the protein truncation assay, and DNA sequencing. A change in DNA methylation status is commonly observed in cancer, and specific methodology for methylation analysis is also provided by this volume.
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