Plant Virology Protocols New Approaches to Detect Viruses and Host Responses


Author : Ichiro Uyeda & Chikara Masuta

Published in : Humana press

ISBN : 978-1-4939-1743-3

File Type : pdf

File Size : 9 mb

Language : English


Recent developments in genome analyses and cytological technologies have made noble approaches to solving many problems in plant virology. The techniques have been applied to not only diagnostic studies of virus diseases but also ecology and pathogenicity of viruses and host responses to viral infection. The first six chapters describe techniques relevant to the detection of unknown viruses and disease diagnosis. Shimura et al. describe a simple and practical diagnostic method of macroarray. Diagnostic methods using PCR are presented by MacKenzie et al. for Potato virus X , Gabino for grapevine viruses, Sasaya for rice viruses, and Noris and Miozzi for Tomato yellow leaf curl Sardinia virus . Atsumi et al. describe the dsRNA extraction method for isolating viruses of unknown sequences. The next nine chapters deal with utilization of meta-genome sequencing and global gene expression analyses for the search and identifi cation of viruses, as well as the elucidation of host responses to viral infection. They also describe analyses of huge sequence data. The methods have potential to detect undescribed viruses and network of host responses not yet known. Kondo et al. look for non-retroviral RNA viruslike sequence in plant genome. Nagano et al. describe sequencing of RNAs by next-generation sequencers to detect viruses in wild plants. Wang et al. describe the cloning and profi le analysis of satsiRNAs from satRNAs of Cucumber mosaic virus . Miozzi and Pantaleo describe analyses of the huge amount of data collected from viral short interfering RNA sequencing. Motooka et al. detected many viral sequences of plant and algal and protozoal viruses in addition to bacteriophage, mammal, and insect-derived viruses in fecal samples from cows by high throughput sequencing. Adkar-Purushothama et al. describe a deep sequencing of viroid infected plants and analyses of viroid-specifi c small RNAs. Ezawa et al. describe extraction of total dsRNAs from fungi, and their cDNAs are sequenced and searched for viral sequence. Matsuura et al. describe the high-throughput Super SAGE for host and viral gene expression. Ishihara et al. describe the use of global gene expression analyses for elucidating the complex network for resistance to viruses.
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