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Published in : ACADEMIC PRESS
ISBN : 0-12-002622-8
File Type : pdf
File Size : 14 mb
Language : English
Description
Over the past ten years, the immobilization of enzymes has been the subject of increased interest, and a number of papers on transformations of organic compounds by immobilized enzymes have been published. Among these many studies, in 1969 we (Tosa et al., 1966, 1967; Chibata et al., 1972) first succeeded in the industrial application of immobilized enzyme, i.e., immobilized aminoacylase, for continuous production of L-amino acids from acyl-DL-amino acids. Succeeding to this industrialization, productions of 6-aminopenicillanic acid (6-APA) by immobilized penicillin amidase and fructose by immobilized glucose isomerase are said to be industrially operated in the United States, Europe, and Japan. Further, very recently a number of papers have been published on the immobilization of microbial cells for the purpose of transformations of organic compounds. In this field, we also studied the immobilization of whole microbial cells-Escherichia coli having high aspartase activity-and in 1973 succeeded in industrial application of these immobilized cells for continuous production of L-aspartic acid from ammonium fumarate (Chibata et al., 1974e; Tosa et al., 1974). This is also the first industrial application of immobilized microbial cells in the world. Further, in 1974 we (Chibata et al., 1975~; Yamamoto et al., 1976, 1977) succeeded in industrial production of L-malic acid from fumarate using immobilized Breuibacteriurn arnrnoniagenes having high fumarase activity.
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