Bacterial and Bacteriophage Genetics 5th Edition (PDF)

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Bacterial and Bacteriophage Genetics

Author: Edward A. Birge
Release at: 2006
Pages: 582
Edition: 5th Edition
File Size: 9 MB
File Type: PDF
Language: English




Description of Bacterial and Bacteriophage Genetics 5th Edition (PDF)

Bacterial and Bacteriophage Genetics 5th Edition by Edward A. Birge is a great book for bacterial & Bacteriophage genetics available in PDF download. Before beginning the formal study of the genetics of bacteria and their viruses, it is important to understand the fundamentals of microbial cell, chromosome, and DNA structures, and the basic viral processes. This chapter briefly reviews cell anatomy as well as genetic terminology and processes with respect to bacteria, yeast, and viruses and attempts to ensure that all students have the necessary background information for subsequent chapters. Detailed coverage of major genetic processes is provided later in this book.

Genetic investigations and manipulations of bacteria and bacteriophage have made vital contributions to our basic understanding of living cells and to the development of molecular biology and biotechnology. This volume is a survey of the genetics of bacteria and their viruses, and it provides students with a comprehensive introduction to this rapidly changing subject. The book is written for upper level undergraduates and beginning graduate students, particularly those who have had an introductory genetics course.

The 5th edition of Bacterial and Bacteriophage Genetics has been extensively revised to reflect recent advances in the field. The book now has a reader-friendly look, with end-of-chapter questions, "Thinking Ahead" and "Applications" boxes to challenge students’ comprehension and insights. A complete glossary of commonly used terms has been revised and expanded.




Content of Bacterial and Bacteriophage Genetics 5th Edition (PDF)


Chapter 1: Fundamentals of Bacterial and Viral Genetics

  • Nomenclature
  • Prokaryotic Cells and Eukaryotic Cells
  • Structure
  • Ploidy
  • DNA Structure
  • Chromosome Structure
  • Eukaryotes
  • Prokaryotes
  • Selection: An Essential Element of Microbial Genetics
  • Major Genetic Transfer Processes Observed in Microorganisms and Their Viruses
  • Genetic Transformation
  • Transduction
  • Conjugation
  • Protoplast Fusion
  • Electroporation
  • Bacteriophage Genetic Exchange
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized
  • Reference Books for Specific Organisms

    • Chapter 2 :Replication and Analysis of DNA

  • DNA Replication
  • Linking DNA in Arbitrary Ways
  • Enzymology of DNA Restriction and Modification
  • Use of Restriction Fragments to Make New DNA Molecules
  • Analytical Techniques for DNA Molecules
  • Electrophoresis
  • Atomic Force Microscopy
  • Detection of Specific DNA Sequences
  • Polymerase Chain Reaction
  • DNA Sequence Analysis
  • Custom Synthesis of DNA
  • Footprinting
  • Gel Mobility Shift Analysis
  • Yeast Two-Hybrid Systems
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Chapter 3 :Mutations and Mutagenesis

  • Bacterial Variation
  • Plate Spreading
  • Replica Plating
  • Fluctuation Test
  • Measurement of Mutation Rate
  • Expression and Selection of Mutant Cell Phenotypes
  • Expression
  • Selection
  • Genetic Code
  • Kinds of Mutation
  • Base Substitutions
  • Insertion and Deletion Mutations
  • Frameshift Mutations
  • Suppressors of Mutations
  • Mutagens
  • Radiation
  • Chemical Modifiers
  • Base Analogs
  • Cross-Linking Agents
  • Transposons
  • Mutator Mutations
  • Site-Directed Mutagenesis
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Chapter 4 :Transcription and Translation: Processes and Basic Regulation

  • RNA Structure
  • Transcription
  • RNA Processing
  • Translation of the Genetic Message
  • Ribosome Function
  • Protein Folding
  • Regulation
  • Examples of Regulation in Simple Functional Units
  • Operon: The Basic Regulatory Unit
  • Lactose Operon
  • Galactose Operon
  • Tryptophan Operon
  • Summary
  • Questions for Review and Discussion
  • Suggestions for Further Reading
  • General
  • Specialized

    • Chapter 5 :DNA Repair and Simple Recombination

  • DNA Repair
  • Mismatch Repair: An Example of Intrareplicational Repair
  • Ultraviolet-Irradiated DNA as a Model Repair System
  • The SOS Global Regulatory Network
  • A Conceptual Model for Generalized Recombination
  • Genetic and Functional Analysis of Recombination
  • RecA and Similar Proteins
  • Other Genes Affecting Recombination
  • A Model for General Recombination
  • Interrelation of Repair and Recombination Pathways
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Chapter 6 :T4 Bacteriophage as a Model Genetic System

  • Morphology and Composition
  • Experimental Methods Used to Study Phage Infection
  • Genetic Organization of T4
  • Types of Mutations Observed in the T4 Genome
  • Genetic Crosses
  • Visconti–Delbrück Analysis
  • Phage Heterozygotes
  • DNA Replication
  • Molecular Biology of T4 Phage Infection
  • Initial Stages of Infection
  • Regulatory Issues
  • Morphogenesis and Maturation
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Chapter 7 :Genetics of Other Intemperate Bacteriophages

  • Other Members of the T Series
  • Bacteriophages T2 and T6
  • Bacteriophage T1
  • Bacteriophage T5
  • Bacteriophages T7 and T3
  • Bacteriophages Containing Single-Strand DNA
  • Bacteriophages Belonging to the Ff Group
  • Bacteriophage φX174
  • RNA-Containing Bacteriophages
  • Single-Stranded RNA Viruses
  • Double-Stranded RNA Viruses
  • Bacteriophages Infecting Bacillus subtilis
  • Bacteriophage SP01
  • Bacteriophage φ29
  • Bacteriophages Infecting the Archaea
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Chapter 8 :Genetics of Temperate Bacteriophages

  • General Nature of the Temperate Response
  • Bacteriophage Lambda as the Archetypal Temperate Phage
  • Lytic Life Cycle
  • Temperate Life Cycle
  • Genetic Map of Lambda
  • Other Lambdoid Phages
  • Bacteriophage P22
  • Bacteriophages P2 and P4
  • Bacteriophage P1
  • Bacteriophage Mu
  • SSV1 and SSV2
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Chapter 9 :Transduction

  • Bacteriophage Lambda: A Specialized Transducing Phage
  • Production of Transducing Particles
  • Physiology and Genetic Consequences of Transduction
  • Lambda Phages That Transduce Additional Genetic Markers
  • Specialized Transducing Phages Other than Lambda
  • Bacteriophage φ80
  • Bacteriophage P1
  • Bacteriophage P22
  • Generalized Transduction
  • Bacteriophage P22
  • Bacteriophage P1
  • Other Phages
  • Analysis of Transductional Data
  • Generalized Transduction
  • Specialized Transduction
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Chapter 10: Genetic Transformation

  • Standard Genetic Transformation Systems
  • Discovery of Genetic Transformation
  • Competent Cells
  • DNA Uptake and Entry
  • Establishment of Donor DNA in the Recipient Cell
  • Other Transformation Systems
  • Escherichia coli
  • Electroporation
  • Transfection
  • E. coli as a Recipient
  • B. subtilis as a Recipient
  • Genetic Mapping Using Transformation
  • Analysis of Transformational Data
  • Genetic Map for B. subtilis
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Chapter 11: Conjugation and the Escherichia coli Paradigm

  • Basic Properties of the E. coli Conjugation System
  • Discovery of Conjugation
  • Discovery of Efficient Donor Strains and Partial Transfer
  • Nature of the Transfer Process
  • Recombination Following Conjugation
  • Construction of the First Genetic Map
  • Interactions of the F Plasmid with the Bacterial Chromosome
  • Integration of the F Plasmid
  • Excision of the F Plasmid
  • Physiology of Conjugation
  • Formation of Mating Aggregates
  • Transfer DNA Synthesis
  • Analysis of the F Plasmid
  • General Structure
  • Genetic Analysis of the F Plasmid
  • Something Completely Different—Possible Diploidy
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Chapter 12: Plasmids and Conjugation Systems Other than F

  • Major Chromosome-Mobilizing Plasmids
  • Salmonella
  • Pseudomonas
  • Enterococcus
  • Streptomyces
  • Agrobacterium
  • Sulfolobus
  • Bacteriocins
  • General Properties
  • Colicins: The Best-Studied Bacteriocins
  • Conjugative Colicin Plasmids
  • Nonconjugative Colicin Plasmids
  • Resistance Plasmids
  • General Properties
  • Plasmid R100
  • Genetic Analysis of Plasmid R100
  • Conjugal Plasmid Interactions
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Chapter 13: Plasmid Molecular Biology

  • Plasmid DNA Replication
  • Interactions Between Plasmid and Host
  • DNA Replication Processes
  • Control of Copy Number
  • Partitioning
  • Incompatibility
  • Conjugal Functions
  • Pilus Production
  • Transfer DNA Replication
  • Broad Host Range Plasmids
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Chapter 14: Advanced Regulatory Topics

  • Regulons
  • Maltose
  • Heat Shock Proteins
  • Global Regulatory Networks
  • Nitrogen Regulation
  • Endospore Formation in Bacillus
  • Inteins
  • Small RNA Molecules
  • Summary
  • Questions for Review and Discussion
  • References
  • Generalized
  • Specialized

    • Chapter 15: Site-Specific Recombination

  • General Principles
  • Double Site-Specific Recombination
  • Phage Lambda Integration
  • Circular Chromosome Segregation
  • Inversion Systems
  • Integrons
  • Transposons: Single Site-Specific Recombination
  • Transposon Tn10
  • Phage Mu Transposition
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Chapter 16: Applied Bacterial Genetics

  • More Information About Recombinant DNA Technology
  • Restriction Mapping
  • Sources of DNA for Linking
  • Safety Considerations
  • Difficulties with the Expression of Cloned DNA
  • Cloning Vectors
  • Phage λ Vectors
  • Phage M13 Vectors
  • Expression Vectors
  • Broad Host Range Vectors
  • Runaway Replication Vectors
  • Artificial Chromosomes
  • Applications of DNA Cloning
  • Peptide Display Systems
  • Laboratory Reagents
  • Phage as Therapeutic Agents
  • Other Applications of Bacterial Genetics
  • Mutagenicity Testing
  • Recombineering
  • Genetic Transfer in Nature
  • Ecogenomics
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Chapter 17: Bacterial and Bacteriophage Evolution

  • What Is Evolution?
  • Implications of Strain Differences
  • Cryptic Genes
  • Expression of Evolutionary Relationships
  • Genomics and Proteomics
  • Bacteria
  • Bacteriophage
  • Specific Examples of Evolution
  • Evolution of Genomes
  • Evolution of the Genetic Code
  • Evolution of Proteins
  • Evolution of Regulatory Sequences
  • Evolution of Mitochondria and Other Endosymbionts
  • Genetic Structure of the Chromosome
  • Bacteria
  • Bacteriophage
  • Summary
  • Questions for Review and Discussion
  • References
  • General
  • Specialized

    • Appendix 1 Laws of Probability and Their Application to Prokaryote Cultures

    Appendix 2 Useful World Wide Web Sites for Bacterial Genetics

    Appendix 3 Answers to Application Questions

    Glossary

    Index

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