Biomolecular Sensors 1st Edition (PDF)

Biomolecular Sensors

Electra Gizeli, Christopher R. Lowe
Release at: 2002
Pages: 337
First Edition
File Size: 3 MB
File Type: pdf
Language: English

Description of Biomolecular Sensors 1st Edition (PDF)

Biomolecular Sensors 1st Edition written by Electra Gizeli, Christopher R. Lowe is a great book available for (PDF) download. The recent substantial advances in the understanding of the genomes of pro- and eukaryotic organisms which underpin growth, expression, differentiation, and productivity have far-reaching consequences in pure, strategic and applied science. The “-omics” triad of genomics, proteomics, and metabolomics are beginning to enable scientists to establish outline mechanistic descriptions of the key bioinformatic drivers, the metabolic pathways, and the accumulation and/or assembly of components leading to all classes of end-product.

The development of new therapeutic drugs ranks among the most laborious and capital intensive of all mankind’s activities. Recent estimates of the number of individual genes in the human genome (~30,000) and the number of unique chemical structures theoretically attainable using existing chemistries (~108) suggest that up to 1012 assays would be required to map the structure-activity space for all potential therapeutic targets. However, this need for specific, well-validated molecular targets and the unwieldy scale and duration of drug development are now being addressed by these new “-omics” disciplines.

Content of Biomolecular Sensors 1st Edition (PDF)

PART I: Biological recognition

Chapter 1: Principles of antigen-antibody recognition

  • Introduction
  • Structure of immunoglobulins
  • Structure of paratopes
  • Structure of epitopes
  • Antigen-antibody interactions
  • The specificity of immunological reagents
  • Structure-function relationships in immunochemistry
  • References
  • Chapter 2: Protein-protein interactions

  • Characterization of protein-protein interfaces
  • Double mutant cycle analysis of a protein-protein interface
  • Mutational induced structural rearrangement in protein interfaces
  • Kinetic pathway of protein-protein association
  • The mechanism of association of a protein complex
  • Measuring protein-protein interactions inhomogeneous and heterogeneous phase
  • Affinity versus activity
  • Summary
  • References
  • Chapter 3: DNA interactions

  • Analog recognition
  • Middle of the spectrum
  • Digital recognition
  • References
  • PART II: Immobilization of biomolecules

    Chapter 4: Immobilization chemistry of biological recognition molecules

  • Introduction
  • Creation of the molecular recognition interface on the transducer
  • The transducer surface
  • Adsorption to the transducer surface
  • Entrapment methods
  • Covalent coupling chemistry
  • Techniques giving some control over the orientation of the immobilized antibody
  • Spatial control of surface immobilization
  • Conclusion
  • References
  • Chapter 5: Binding isotherms and kinetics of immobilized biological systems

  • Introduction
  • The determination of binding constants and of kinetic rate constants
  • Conclusions
  • References
  • PART III: Transducer technology

    Chapter 6: Optical transducers

  • Introduction
  • Optical transducers: general considerations
  • Direct detection
  • Fluorescence transducers
  • Conclusions and outlook
  • Acknowledgments
  • References
  • Chapter 7: Acoustic transducers

  • Introduction
  • Elastic waves in solids
  • Acoustic wave devices
  • Acoustic wave sensors for studying biomolecular interactions
  • Comparison of acoustic sensors
  • Conclusions
  • Acknowledgments
  • References
  • Chapter 8: Immunoassays using enzymatic amplification electrodes

  • Introduction
  • Coupling of immunoassays with enzymatic recycling electrodes
  • Conclusions
  • Acknowledgment
  • References
  • PART IV: Applications

    Chapter 9: Surface plasmon resonance: development and use of BIACORE instruments for

  • biomolecular interaction analysis
  • Introduction
  • Application demands
  • SPR-refractometer instrumental configurations
  • Sensor surface chemistry
  • Biomolecular interaction analysis-BIA
  • Marketing
  • References
  • Chapter 10: Sys: the resonant mirror biosensor

  • Introduction
  • Modus operandi – light and surfaces
  • Developments in kinetics
  • Recent applications
  • Conclusion
  • References
  • Chapter 11: Commercial quartz crystal microbalances: theory and applications

  • Introduction
  • Quartz crystal microbalance – theory
  • Commercial systems
  • Applications
  • Conclusion and future directions
  • Bibliography
  • Chapter 12: The quartz crystal microbalance with dissipation monitoring (QCM-D)

  • Introduction
  • The QCM beyond the Sauerbrey regime
  • Application examples
  • Example 1: DNA
  • Example 2: Mussel adhesive protein
  • Concluding remarks
  • Appendix
  • References
  • Index


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