Biomolecular Sensors 1st Edition (PDF)

Author:	Electra Gizeli, Christopher R. Lowe
Release at:	2002
Pages:	337
Edition:	First Edition
File Size:	3 MB
File Type:	pdf
Language:	English

Description of Biomolecular Sensors 1st Edition (PDF)

Biomolecular Sensors 1st Edition written by Electra Gizeli, Christopher R. Lowe is a great book available for (PDF) download. The recent substantial advances in the understanding of the genomes of pro- and eukaryotic organisms which underpin growth, expression, differentiation, and productivity have far-reaching consequences in pure, strategic and applied science. The “-omics” triad of genomics, proteomics, and metabolomics are beginning to enable scientists to establish outline mechanistic descriptions of the key bioinformatic drivers, the metabolic pathways, and the accumulation and/or assembly of components leading to all classes of end-product.

The development of new therapeutic drugs ranks among the most laborious and capital intensive of all mankind’s activities. Recent estimates of the number of individual genes in the human genome (~30,000) and the number of unique chemical structures theoretically attainable using existing chemistries (~108) suggest that up to 1012 assays would be required to map the structure-activity space for all potential therapeutic targets. However, this need for specific, well-validated molecular targets and the unwieldy scale and duration of drug development are now being addressed by these new “-omics” disciplines.

Content of Biomolecular Sensors 1st Edition (PDF)

PART I: Biological recognition

Chapter 1: Principles of antigen-antibody recognition

Introduction

Structure of immunoglobulins

Structure of paratopes

Structure of epitopes

Antigen-antibody interactions

The specificity of immunological reagents

Structure-function relationships in immunochemistry

References

Chapter 2: Protein-protein interactions

Characterization of protein-protein interfaces

Double mutant cycle analysis of a protein-protein interface

Mutational induced structural rearrangement in protein interfaces

Kinetic pathway of protein-protein association

The mechanism of association of a protein complex

Measuring protein-protein interactions inhomogeneous and heterogeneous phase

Affinity versus activity

Summary

References

Chapter 3: DNA interactions

Analog recognition

Middle of the spectrum

Digital recognition

References

PART II: Immobilization of biomolecules

Chapter 4: Immobilization chemistry of biological recognition molecules

Introduction

Creation of the molecular recognition interface on the transducer

The transducer surface

Adsorption to the transducer surface

Entrapment methods

Covalent coupling chemistry

Techniques giving some control over the orientation of the immobilized antibody

Spatial control of surface immobilization

Conclusion

References

Chapter 5: Binding isotherms and kinetics of immobilized biological systems

Introduction

The determination of binding constants and of kinetic rate constants

Conclusions

References

PART III: Transducer technology

Chapter 6: Optical transducers

Introduction

Optical transducers: general considerations

Direct detection

Fluorescence transducers

Conclusions and outlook

Acknowledgments

References

Chapter 7: Acoustic transducers

Introduction

Elastic waves in solids

Acoustic wave devices

Acoustic wave sensors for studying biomolecular interactions

Comparison of acoustic sensors

Conclusions

Acknowledgments

References

Chapter 8: Immunoassays using enzymatic amplification electrodes

Introduction

Coupling of immunoassays with enzymatic recycling electrodes

Conclusions

Acknowledgment

References

PART IV: Applications

Chapter 9: Surface plasmon resonance: development and use of BIACORE instruments for

biomolecular interaction analysis

Introduction

Application demands

SPR-refractometer instrumental configurations

Sensor surface chemistry

Biomolecular interaction analysis-BIA

Marketing

References

Chapter 10: Sys: the resonant mirror biosensor

Introduction

Modus operandi – light and surfaces

Developments in kinetics

Recent applications

Conclusion

References

Chapter 11: Commercial quartz crystal microbalances: theory and applications

Introduction

Quartz crystal microbalance – theory

Commercial systems

Applications

Conclusion and future directions

Bibliography

Chapter 12: The quartz crystal microbalance with dissipation monitoring (QCM-D)

Introduction

The QCM beyond the Sauerbrey regime

Application examples

Example 1: DNA

Example 2: Mussel adhesive protein

Concluding remarks

Appendix

References

Index

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