Molecular Cloning A Laboratory Manual 3rd Edition (PDF)

Molecular Cloning A Laboratory Manual

Author: Joseph Sambrook, David W. Russel
Release at: 2001
Pages: 2231
Edition: 3rd Edition (Three Volume Set)
File Size: 123 MB
File Type: PDF
Language: English



Description of Molecular Cloning A Laboratory Manual 3rd Edition (PDF)

Molecular Cloning A Laboratory Manual 3rd Edition by Joseph Sambrook, D. W. Russel is a great laboratory manual book available for PDF download. The 1st edition of this manual was written some 20 years ago, when basic methods in molecular cloning were far from robust and were established in only a few laboratories. The appearance of that book did much to change the picture. The manual's didactic character gave readers onfidence to use techniques that still seemed magical during the late 1970s and early 1980s. The second edition, published just 7 years after the first, was smoother in style and far richer in reliable content. By then, individual methods had become more durable and portable, but it was still difficult to string them successfully together into multistep procedures. This new edition, almost certainly the last to appear in book form, reflects a mature discipline working at full power and with high reliability. During the 1990s, many of the drearier, more repetitive techniques of molecular biology have been automated; demanding, multistep procedures have been converted into kits; high-quality genomic and cDNA libraries are now available from the shelves of commercial manufacturers; and all manipulations involving nucleic acids have benefited greatly from improvements in the quality of reagents and enzymes. As a consequence of these and other advances, competent laboratory workers can now easily avoid experimental problems that beset even the best investigators just a few years ago. This is not to say that everything works perfectly all of the time or that no further improvement is possible. However, difficulties now can largely be avoided by careful planning and application of existing knowledge rather than by experimental trial and error.

A major goal of all three editions of Molecular Cloning has been to provide researchers with up-to-date protocols that work reproducibly. Users of the previous editions will recognize many of the organizational features in the experimental sections of this book. Nevertheless, the revision of the text has been extensive and detailed. Ancient protocols have been modernized, while new protocols have been added to reflect the continuing penetration of molecular cloning into almost all areas of biomedical research. Of equal importance has been our desire to explain how and why particular methods work, and with reasoned arguments for choosing between alternative procedures. This edition therefore contains not only annotations at crucial points in the protocols, but also an abundance of material in the form of Information Panels, which are placed at the end of the chapters as well as in Appendix 9. We hope that these 115 panels spread throughout the three volumes of the book will provide clear insights into the reasons why methods are carried out in a certain manner and how techniques have progressively evolved. Finally, we have provided extensive references to the scientific literature so that curious readers can trace methods and ideas to their roots. Few will read this book from beginning to end. But we hope that the community of cloners will find in these pages much to stimulate the mind and to facilitate the work of their hands.




Content of Molecular Cloning A Laboratory Manual 3rd Edition (PDF)


Volume 1

Chapter 1: Plasmids and their Usefulness in Molecular Cloning

Chapter 2: Bacteriophage λ and its Vectors

Chapter 3: Working with Bacteriophage M13 Vectors

Chapter 4: Working with High-capacity Vectors

Chapter 5: Gel Electrophoresis of DNA and Pulsed-Agrose Gel Electrophoresis

Chapter 6: Preparation and Analysis of Eukaryotic Genomic DNA

Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells

Volume 2

Chapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction

Chapter 9: Preparation of Radiolabeled DNA and RNA Probes

Chapter 10: Working with Synthetic Oligonucleotide Probes

Chapter 11: Preparation of cDNA Libraries and Gene Identification

Chapter 12: DNA Sequencing

Chapter 13: Mutagenesis

Chapter 14: Screening Expression Libraries

Volume 3

Chapter 15: Expression of Cloned Genes in Escherichia coli

Chapter 16: Introducing Cloned Genes into Cultured Mammalian Cells

Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells

Chapter 18: Protein Interaction Technologies

Appendices

Index

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