Practical Biochemistry

Practical Biochemistry
 
Author:
Geetha Damodaran K
Publisher: JAYPEE BROTHERS MEDICAL
ISBN No: 978-93-5025-141-6
Release at: 2011
Pages: 242
Edition:
First Edition
File Size:24MB
File Type: pdf
Language: English



Description of Practical Biochemistry

Nearly two decades of teaching experience have driven me to write this book. I realized that if an illustrated book is available, students will be able to recollect the experiments done earlier, to face the different types of questions during practical examinations. Hence all the items in this book are illustrated.
The contents of this book are structured in a practical examination-oriented manner. The major sections are qualitative experiments, quantitative experiments, charts, spotters and objective structured practical examination questions. All the tests are provided with diagrams and interpretations. This will help the students to understand each concept thoroughly and enable them to use it as an instant doubt clearing book. I hope it will be very useful for day-to-day studies and exam preparations.
Details of reagent preparations given along with the respective chapters are useful for the staff involved in the laboratory preparation of practical sessions. This part will also help to improve the level of understanding of students about the reagents they are using for various experiments in the laboratory.
Questions provided with the chapters are useful for having better clarity and grasp of the topic. Moreover, it will definitely boost the confidence of students to face the examination. Chapters on charts and spotting and OSPE questions are useful for the self-training of such types of evaluation methods. I warmly welcome the views of those using the book and I shall be grateful to the readers for bringing to my notice of mistakes for corrections, in future editions of the book.

Content of Practical Biochemistry


SECTION ONE: QUALITATIVE ANALYSIS
1. Reactions of Carbohydrates ........................................................................................................... 3
2. Reactions of Proteins ..................................................................................................................... 18
3. Reactions of Lipids ........................................................................................................................ 37
4. Reactions of Urea ........................................................................................................................... 42
5. Reactions of Creatinine ................................................................................................................. 45
6. Uric Acid ......................................................................................................................................... 47
7. Scheme for Identification of Biologically Important Substance in a Given  solution ........... 50
8. Urine Analysis ............................................................................................................................... 51
9. Spectroscopy .................................................................................................................................. 65
10. Reactions of Milk ........................................................................................................................... 72

SECTION TWO: QUANTITATIVE ANALYSIS

11. Principles of Colorimetry ............................................................................................................. 77
12. Determination of Blood Sugar ..................................................................................................... 83
13. Determination of Urea .................................................................................................................. 90
14. Determination of Creatinine ........................................................................................................ 94
15. Determination of Total Protein and Albumin ........................................................................... 98
16. Determination of Cholesterol .................................................................................................... 102
17. Determination of Uric Acid ....................................................................................................... 105
18. Determination of Bilirubin ......................................................................................................... 109
19. Determination of Transaminases .............................................................................................. 113
20. Determination of Alkaline Phosphatase .................................................................................. 119
21. Determination of Calcium .......................................................................................................... 122
22. Determination of Phosphorus ................................................................................................... 127
23. Determination of Titrable Acidity and Ammonia in Urine .................................................. 131
24. Determination of Urine Chloride .............................................................................................. 135

SECTION THREE: CHARTS

25. Charts ............................................................................................................................................ 141


SECTION FOUR: SPOTTERS

26. Spotters ......................................................................................................................................... 173
SECTION FIVE: OSPE (OBJECTIVE STRUCTURED PRACTICAL EXAMINATION)

QUESTIONS

27. OSPE (Objective Structured Practical Examination) Questions ........................................... 213
Index ............................................................................................................................................................ 225
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