| Author: | Florence G Burleson, Thomas M. Chambers & Danny L. Wiedbrauk |
| Release at: | 1992 |
| Pages: | 243 |
| Edition: | First Edition |
| File Size: | 14 MB |
| File Type: | |
| Language: | English |
Description of Virology: A Laboratory Manual 1st Edition (PDF)
Virology A Laboratory Manual 1st Edition by Florence G Burleson, Thomas M. Chambers & Danny L. Wiedbrauk is a great virology book available in (eBook) PDF download. This text was originally developed for a one-semester virology laboratory course. It is designed for the classroom with detailed experimental protocols and comprehensive lists of the materials required for each experiment. Since it is not possible to include all virological techniques in a single volume, the selected experiments are those that best illustrate key points of basic virology. The experiments are also designed to minimize the need for extensive preparation or sophisticated instrumentation. Our aim in preparing this text is not a complete treatise of virological methods, but the development of a practical and sequentially organized series of experiments.
More specifically, this laboratory text is designed to: (1) introduce the student to cell culture through a series of experiments that illustrate the basic principles involved in the routine passage and cryopreservation of cell lines; (2) demonstrate how to passage and preserve a virus stock in the laboratory; (3) assay and quantify the virus stocks produced in a number of different assay systems, in order to better appreciate what a given assay measures, its relative sensitivity, and its relationship to other virus assays; (4) introduce some of the simpler techniques in identifying a virus; (5) assay for viral antibody and perform immunofluorescent staining of virus-infected cells; (6) integrate many of the techniques previously mastered through the production and quantification of interferons; and (7) demonstrate some of the basic techniques of molecular biology that are now commonly used in the virology laboratory.
In this text, a given exercise not only demonstrates a principle of cell culture or virology, but also builds upon a previous experiment. This sequential organization, however, still allows for flexibility. Many more experiments are included than could be attempted in a normal semester, thus providing the instructor with choices within this sequential framework. The use, throughout this text, of only three cell lines and two viruses provides continuity and simplifies the preparation of the laboratory exercises.
Content of Virology: A Laboratory Manual 1st Edition (PDF)
Chapter 1: INTRODUCTION TO THE VIROLOGY LABORATORY
Chapter 2: INTRODUCTION TO CELL CULTURE
Chapter 3: PREPARATION OF CELL CULTURE MEDIUM
Chapter 4: CELL GROWTH AND CELL PASSAGE
Chapter 5: COUNTING CELLS WITH A HEMACYTOMETER
Chapter 6: PRIMARY CELL CULTURES
Chapter 7: CRYOPRESERVATION OF CELLS
Chapter 8: INTRODUCTION TO VIRUS PASSAGE
Chapter 9: VIRUS REPLICATION IN CELL CULTURE
Chapter 10: VIRUS REPLICATION IN EMBRYONATED EGGS
Chapter 11: INTRODUCTION TO QUANTAL VIRUS ASSAYS
Chapter 12: TCID50
Chapter 13: LD50
Chapter 14: EIDso
Chapter 15: INTRODUCTION TO QUANTITATIVE ASSAYS
Chapter 16: PLAQUE ASSAYS
Chapter 17: ASSAYS OF OTHER VIRUS PROPERTIES
Chapter 18: HEMAGGLUTINATION ASSAY
Chapter 19: HEMADSORPTION
Chapter 20: INTRODUCTION TO VIRUS CHARACTERIZATION
Chapter 21: VIRAL REPLICATION: ONE-STEP GROWTH CURVE
Chapter 22: DETERMINATION OF VIRUS SIZE
Chapter 23: ASSESSING THERMOSTABILITY
Chapter 24: SENSITIVITY TO LIPID SOLVENTS
Chapter 25: SENSITIVITY TO LOW pH
Chapter 26: INTRODUCTION TO TECHNIQUES FOR DETECTION OF VIRAL ANTIBODY
Chapter 27: ENCEPHALOMYOCARDITIS VIRUS NEUTRALIZATION
Chapter 28: HEMAGGLUTINATION-INHIBITION ASSAY
Chapter 29: IMMUNOFLUORESCENT STAINING OF EMC VIRUS-INFECTED CELLS
Chapter 30: ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)
Chapter 31: ASSAYS OF ANTIVIRAL AGENTS AND INTERFERONS
Chapter 32: PRODUCTION OF INTERFERON
Chapter 33: PLAQUE-REDUCTION BIOASSAY
Chapter 34: CYTOPATHIC EFFECT INHIBITION BIOASSAY
Chapter 35: HEMAGGLUTININ (HA) YIELD - REDUCTION BIOASSAY
Chapter 36: RADIOIMMUNOPRECIPITATION
Chapter 37: WESTERN BLOT ANALYSIS OF EMC VIRUS PROTEINS
Chapter 38: ISOLATION OF VIRAL NUCLEIC ACIDS FROM VIRIONS
Chapter 39: ISOLATION OF VIRAL NUCLEIC ACIDS FROM INFECTED CELLS
Chapter 40: ANALYSIS OF VIRAL NUCLEIC ACIDS BY GEL ELECTROPHORESIS
APPENDIX A: Viruses and Cell Lines
APPENDIX B: Media and Solutions
APPENDIX C: Procedures and Calculations
INDEX
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