Virology: A Laboratory Manual 1st Edition (PDF)

Virology A Laboratory Manual

Author: Florence G Burleson, Thomas M. Chambers & Danny L. Wiedbrauk
Release at: 1992
Pages: 243
Edition: First Edition
File Size: 14 MB
File Type: PDF
Language: English




Description of Virology: A Laboratory Manual 1st Edition (PDF)

Virology A Laboratory Manual 1st Edition by Florence G Burleson, Thomas M. Chambers & Danny L. Wiedbrauk is a great virology book available in (eBook) PDF download. This text was originally developed for a one-semester virology laboratory course. It is designed for the classroom with detailed experimental protocols and comprehensive lists of the materials required for each experiment. Since it is not possible to include all virological techniques in a single volume, the selected experiments are those that best illustrate key points of basic virology. The experiments are also designed to minimize the need for extensive preparation or sophisticated instrumentation. Our aim in preparing this text is not a complete treatise of virological methods, but the development of a practical and sequentially organized series of experiments.

More specifically, this laboratory text is designed to: (1) introduce the student to cell culture through a series of experiments that illustrate the basic principles involved in the routine passage and cryopreservation of cell lines; (2) demonstrate how to passage and preserve a virus stock in the laboratory; (3) assay and quantify the virus stocks produced in a number of different assay systems, in order to better appreciate what a given assay measures, its relative sensitivity, and its relationship to other virus assays; (4) introduce some of the simpler techniques in identifying a virus; (5) assay for viral antibody and perform immunofluorescent staining of virus-infected cells; (6) integrate many of the techniques previously mastered through the production and quantification of interferons; and (7) demonstrate some of the basic techniques of molecular biology that are now commonly used in the virology laboratory.

In this text, a given exercise not only demonstrates a principle of cell culture or virology, but also builds upon a previous experiment. This sequential organization, however, still allows for flexibility. Many more experiments are included than could be attempted in a normal semester, thus providing the instructor with choices within this sequential framework. The use, throughout this text, of only three cell lines and two viruses provides continuity and simplifies the preparation of the laboratory exercises.




Content of Virology: A Laboratory Manual 1st Edition (PDF)


Chapter 1: INTRODUCTION TO THE VIROLOGY LABORATORY

Chapter 2: INTRODUCTION TO CELL CULTURE

Chapter 3: PREPARATION OF CELL CULTURE MEDIUM

Chapter 4: CELL GROWTH AND CELL PASSAGE

Chapter 5: COUNTING CELLS WITH A HEMACYTOMETER

Chapter 6: PRIMARY CELL CULTURES

Chapter 7: CRYOPRESERVATION OF CELLS

Chapter 8: INTRODUCTION TO VIRUS PASSAGE

Chapter 9: VIRUS REPLICATION IN CELL CULTURE

Chapter 10: VIRUS REPLICATION IN EMBRYONATED EGGS

Chapter 11: INTRODUCTION TO QUANTAL VIRUS ASSAYS

Chapter 12: TCID50

Chapter 13: LD50

Chapter 14: EIDso

Chapter 15: INTRODUCTION TO QUANTITATIVE ASSAYS

Chapter 16: PLAQUE ASSAYS

Chapter 17: ASSAYS OF OTHER VIRUS PROPERTIES

Chapter 18: HEMAGGLUTINATION ASSAY

Chapter 19: HEMADSORPTION

Chapter 20: INTRODUCTION TO VIRUS CHARACTERIZATION

Chapter 21: VIRAL REPLICATION: ONE-STEP GROWTH CURVE

Chapter 22: DETERMINATION OF VIRUS SIZE

Chapter 23: ASSESSING THERMOSTABILITY

Chapter 24: SENSITIVITY TO LIPID SOLVENTS

Chapter 25: SENSITIVITY TO LOW pH

Chapter 26: INTRODUCTION TO TECHNIQUES FOR DETECTION OF VIRAL ANTIBODY

Chapter 27: ENCEPHALOMYOCARDITIS VIRUS NEUTRALIZATION

Chapter 28: HEMAGGLUTINATION-INHIBITION ASSAY

Chapter 29: IMMUNOFLUORESCENT STAINING OF EMC VIRUS-INFECTED CELLS

Chapter 30: ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)

Chapter 31: ASSAYS OF ANTIVIRAL AGENTS AND INTERFERONS

Chapter 32: PRODUCTION OF INTERFERON

Chapter 33: PLAQUE-REDUCTION BIOASSAY

Chapter 34: CYTOPATHIC EFFECT INHIBITION BIOASSAY

Chapter 35: HEMAGGLUTININ (HA) YIELD - REDUCTION BIOASSAY

Chapter 36: RADIOIMMUNOPRECIPITATION

Chapter 37: WESTERN BLOT ANALYSIS OF EMC VIRUS PROTEINS

Chapter 38: ISOLATION OF VIRAL NUCLEIC ACIDS FROM VIRIONS

Chapter 39: ISOLATION OF VIRAL NUCLEIC ACIDS FROM INFECTED CELLS

Chapter 40: ANALYSIS OF VIRAL NUCLEIC ACIDS BY GEL ELECTROPHORESIS

APPENDIX A: Viruses and Cell Lines

APPENDIX B: Media and Solutions

APPENDIX C: Procedures and Calculations

INDEX

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